KMID : 0379119900180020084
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Korean Journal of Mycology 1990 Volume.18 No. 2 p.84 ~ p.88
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Optimal Material and Conditions for the Immobilization of Rhizopus nigricans in the 11 ¥á-Hydroxylation Reaction of Progesterone
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Abstract
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Hydroxylation in the 11¥á-position of progesterone molecules was carried out using Rhizopus nigricans spores immobilized within various gel matrices, among which polyacrylamide and agar gel were proved to be the most effective. Reactions with the immobilized cells and intact free cells showed almost identical conversion rate of progesterone, optimal pH and reaction time for attaining maximal yield, from which were confirmed absence of any decay and modification of enzyme activities.
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